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Figure 10
Difficulties at low resolution. (a) Higher resolution shows that this is a regular β-strand with no outliers or clashes, but in (b) the backbone CO directions are misoriented because they are not observed, and the side chains are pulled inwards towards density nubbins. Contours are at 1.2σ. (c) Localized outliers for a long sequence misalignment in 70S ribosomal protein L27 at 3.2 Å resolution (PDB entry 3i1n). (d) After rebuilding of the one- to three-residue sequence shifts in the improved PDB entry 4gd1 (Dunkle et al., 2011BB16).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 74| Part 2| February 2018| Pages 132-142
https://doi.org/10.1107/S2059798317009834
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