Figure 6
Molecular-replacement solutions generated by Phaser for PDB entry 1igj solved using 2hfl following gyre and gimble. CC per domain (domain H, H 1–113; domain K, H 113–223; domain L, L 1–106); domain M, L 107–200) is shown coloured by value: > 0.50, green; > 0.40, blue; > 0.30, yellow. With an input r.m.s.d. of 1.0 Å the molecular-replacement solution is no better than the standard molecular-replacement solution (Fig. 4), but for an r.m.s.d. of 2.0 or 3.0 Å the overall CC is 0.45, that of the unperturbed aligned structure (Figs. 2 and 4). |