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Figure 7
PDB entry 2hfl perturbed ±35° in 1° increments from optimal superposition on PDB entry 1igj. (a) The purple and cyan dumbbells pass through the centres of mass of the variable and constant domains, respectively, of each Fab, showing the pseudo-twofold axis. The grey dumbbell shows the axis of rotation, with the residues used to split the domains shown in blue for the light chain and yellow for the heavy chain. (b) shows (a) rotated through 90°. (c) The perturbed structures of Fv shown in ribbon representation, with each perturbation in a different colour, from the same view as (a). (d) shows (c) rotated through 90° from the same view as (b). The figure and perturbed coordinates were generated with PyMOL (DeLano, 2002BB13)

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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