Figure 2
Model-phased RIP difference electron-density maps calculated for the thaumatin X-ray RIP data. (a)–(e) represent increasing dose points (Expo. 2, Expo. 3, Expo. 4, Expo. 5 and Expo. 6, respectively) subtracted from the first data set (Expo. 1). Difference density is shown as a green mesh contoured at 6σ. The disulfide bond between Cys126 and Cys177 shows the highest electron density. (f)–(j) are the same difference maps as (a)–(e) but contoured at −6.5σ in the vicinity of Cys66. |