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![[Figure 3]](di5013fig3mag.jpg)
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Figure 3
Model-phased RIP difference map for the cubic insulin UV-RIP experiment. Positive difference electron density contoured at 6σ is represented as a green mesh surrounding cysteine S atoms. Negative electron-density difference is contoured at −5σ as a red mesh in the vicinity of cysteine S atoms. (a) and (c) are RIP difference maps calculated between data sets Before_UV.1 and After_UV. (b) and (d) are RIP difference maps calculated between data sets Before_UV.1 and Before_UV.2, i.e before UV-light exposure. The X-ray-only difference maps show little evidence of radiation damage, whereas the before UV illumination–post UV illumination difference map shows strong positive peaks at cysteine Sγ positions as well as a new peak appearing near Cys20. |
![[Figure 3]](di5013fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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