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Figure 2
The Zn2+-introduction site within the crystal structures of J30 wt and J30 CCH. (a) In J30 wt, a hydrogen bond connects the side chains of His115 and Tyr143. Without interfering, the Hα3 of Gly114 and the side chain of Ala98 point towards them. (b) The crystal structure of J30 CCH shows the successful residue mutations and Zn2+ incorporation. The metal ion is coordinated by the side chains of Cys98 (2.3 Å distance), Cys114 (2.4 Å), His115 and His143 (both 2.1 Å).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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