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Figure 4
J30 CCH exhibits an increased thermal tolerance compared with the wild-type enzyme and Zn2+-stripped J30 CCH. (a, b) Differential scanning fluorimetry. The standard errors of triplicates are shown. (a) The introduction of Zn2+ increased the calculated Tm by 11.2°C. (b) The thermal tolerance of J30 CCH after incubation with EDTA equaled that of the wild-type enzyme, but could be regained by the addition of Zn2+. (c) Circular dichroism. The secondary-structure elements of the triple mutant withstand considerably higher temperatures than those of J30 wt.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 74| Part 7| July 2018| Pages 702-710
https://doi.org/10.1107/S2059798318006678
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