Figure 6
Enzymatic assays. The increase in thermal tolerance is paralleled by a shift in the catalytic profiles of J30 wt and J30 CCH. The pNPC turnover of both enzyme variants is plotted dependent on reaction temperature and pH, showing an increase in the optimal temperature (contour interval of 0.05). Each data-point triplicate is represented by a diamond, the size of which corresponds to its standard error. |