The impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order

Juers, D.H.; Farley, C.A.; Saxby, C.P.; Cotter, R.A.; Cahn, J.K.B.; Holton-Burke, R.C.; Harrison, K.; Wu, Z.

doi:10.1107/S2059798318008793

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 6
Schematics showing the dependence of the cooling response of the unit-cell components on cryosolvent contraction for (a) tetragonal thermolysin and (b) orthorhombic trypsin. Cell and protein contractions were calculated as described in Fig. 1

. The channel contraction was calculated according to Δ_chan = (Δ_cell − ν_protΔ_prot)/(1 − Δ_prot). The lines shown are linear fits to the data over most of the range.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 74| Part 9| September 2018| Pages 922-938

https://doi.org/10.1107/S2059798318008793

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