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Figure 2
(a) TtGH8 activity was explored on a range of substrates using thin-layer chromatography. Hydrolysis activity can be observed on WAX, RAX, BX and MLX, as well as on the oligosaccharides xylotetraose (X4), xylopentaose (X5) and xylohexaose (X6). Activity analysis of TtGH8 on xylohexaose (b), xylopentaose (c) and xylotetraose (d) was carried out with HPAEC-PAD by substrate depletion. The peak areas observed in the HPAEC-PAD traces were normalized against a fucose internal standard and values for ln(S0/St), where S0 is the substrate-peak area at time 0 and St is the substrate-peak area at time t, were plotted against time. Linear regression analysis was used to calculate kcat/Km (in M−1 min−1).

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ISSN: 2059-7983
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