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Figure 2
Overview of the apparatus for acoustic ejection from non-acoustic labware. For acoustic ejection of protein crystals to take place, the distance between the acoustic transducer in the Echo 550 and the bottom of the crystallization plate must equal one of two possible preset values. To accomplish this, crystallization plates must either be cut and placed on a plate of the correct height (a), be combined with a spacer (b) or be lightly sanded (c). There are no similar limits to the size of the destination plate (d). (a) For initial testing, the Echo 550 was used to harvest crystals from a polypropylene assembly which contained fragments of different commercially available plates (inset). (b) To test the harvesting of crystals grown inside the most promising crystallization phase, it was coupled to a thin slice from an acoustically transparent plate (inset). (c) Finally, intact MiTeGen plates were used to grow and harvest protein crystals by lightly sanding down the edge pedestal (inset). (d) Acoustically harvested crystals were transferred to a pin platform that contained up to 96 micro-meshes. The crystals on the micro-meshes (inset) were then pressure-fitted into a MiTeGen Reusable Base (model B1A-R) and cryocooled.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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