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Figure 7
High-throughput fragment screening. Proteinase K crystals were rapidly screened against a fragment library consisting of 96 chemicals. The total laboratory preparation time was 4 min to set up the crystallization drop, 4 min to eject 96 crystal aliquots, 4 min to combine the drops with 96 screened chemicals and 72 min to cryocool the crystals and place them in pucks (this does not include overnight crystal growth or the 10 min soaking time). Three fragments bound to proteinase K were observed in the electron density (OMIT difference map contoured at 3σ; ligands were omitted from structure refinement). The structure-refinement statistics for bicine (a), N-(2-acetamido)iminodiacetic acid (ADA) (b) and tartrate (c) are shown in Table 2[link].

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 74| Part 10| October 2018| Pages 986-999
https://doi.org/10.1107/S2059798318011506
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