Using sound pulses to solve the crystal-harvesting bottleneck

Samara, Y.N.; Brennan, H.M.; McCarthy, L.; Bollard, M.T.; Laspina, D.; Wlodek, J.M.; Campos, S.L.; Natarajan, R.; Gofron, K.; McSweeney, S.; Soares, A.S.; Leroy, L.

doi:10.1107/S2059798318011506

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 8
The number of crystals harvested from a Bingham fluid remains constant as additional aliquots are successively harvested from a single crystallization drop. The number of crystals in each 25 nl harvested aliquot is shown as a function of the number of successive harvests from a single crystallization well (the overall average was 3.8 ± 0.6 crystals per 25 nl harvest). The crystals in the source plate were grown in a Bingham fluid consisting of 0.15%(w/w) agarose (in addition to the normal crystallization components). The Bingham fluid crystallization protocol is described in Supplementary Fig. S2.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 74| Part 10| October 2018| Pages 986-999

https://doi.org/10.1107/S2059798318011506

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