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Figure 9
Harvested crystals are drawn from the surface layer. Observed movement towards the ejection point for all crystals in the field of view (caused by harvesting a 10 nl aliquot) as a function of the initial crystal depth. The movement of each crystal was determined by comparing two successive generated three-dimensional images of the crystal column suspended below the harvesting point (with the 10 nl harvest between them). Deep-dwelling crystals remained largely stationary during serial crystal harvesting, with the majority of the harvested crystals originating from surface layers that moved quickly towards the harvesting point. The data are fitted by a second-order polynomial (y = 520x2 + 70x; one outlier was removed).

Journal logoSTRUCTURAL
ISSN: 2059-7983
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