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Figure 2
Size-exclusion chromatography shows monodisperse OmpF–amphipol A8-35 complexes. (a) Elution profile of monodisperse OmpF–amphipol A8-35 complexes at a 1:10(w:w) ratio. SEC was carried out at a flow rate of 0.5 ml min−1 using a Superose 12 column equilibrated with 20 mM sodium phosphate pH 7.9, 100 mM NaCl. V0 represents the void volume of the column where aggregated proteins elute. Free APol is predicted to elute at 12 ml. (b) The proposed models of OmpF (red; PDB entry 2omp) in amphipol A8-35 and detergent micelles (blue). The OmpF structure is from the PDB with schematics of surrounding APol and detergent micelles. |
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