Figure 4
Model of the distribution of Apol in filaments. (a) The combination of fitted models required to fit the pure Apol scattering in 23.5% D2O. Core/shell tube data (dark blue dashed line) are combined with an oblate sphere model component (cyan dashed line) to provide a fit (red line) to the original data points. (b) Transmission electron microscopy image of OmpF–Apol filaments prepared as in Arunmanee et al. (2014), showing an absence of visible free Apol. (c) Schematic of the sequence of events leading to the formation of filaments and free APol. A variable inter-OmpF distance could explain the lack of long-range structure observed by SANS. |