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Figure 4
ITC binding isotherms for ISG15s and SARS-CoV PLpro. (a) hISG15 with its predicted binding-interface residues with SARS-CoV PLpro colored red and the sites of Phe41 and His39 colored green. (b) Top, representative ITC binding isotherms with the raw heats. Bottom, representative integrated heat peak areas against the molar ratio of ISG15 added to SARS-CoV PLpro. The line shows the best fit to an independent model.

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