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![[Figure 4]](ba5289fig4mag.jpg)
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Figure 4
How HITO works. Schematic diagram of the spatial relationship analyzed by HITO. A cryoloop containing multiple crystals is shown. The rotation axis of the goniometer is horizontal in this figure. HITO regards contiguous `good' pixels on the SHIKA heat map as individual crystals (C1, C2, C3, …). HITO checks overlaps among crystals along the rotation axis. Nonoverlapped crystals are grouped into the `helical full rotation' group (C1). HITO calculates the `vertical' distance (D1, D2, …) between vertically overlapped crystals. For example, if this distance is three times longer than the vertical beam size, the crystal is grouped into the `partial helical' group (C2, C3, C4, C5 and C9 at this stage). Others are grouped into `clustered crystals' (C6, C7 and C8). HITO calculates the crystal size (e.g. S1, S3, S4 and S9). Crystals with smaller sizes along the rotation axis than the `minimum size for helical' are grouped into the `small-wedge' group (C4 and C5). Crystals larger than the `maximum size for helical' are categorized into the `clustered' group (S9). |
![[Figure 4]](ba5289fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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