Figure 2
Examples of crystal-injection methods. The green arrows show the sheath helium flow. (a) Water jet from a GDVN, reproduced from DePonte et al. (2008) with the permission of IOP Publishing. The black arrow shows the flow-focusing area. (b) LCP injector, reproduced from Nogly et al. (2015). The black arrow shows the position of the XFEL beam. (c) Co-flow MESH injection of a photosystem II solution, with a 100 µm inner diameter of the center capillary, adapted from Sierra et al. (2016). Reprinted with permission from Springer Nature. Copyright (2015). (d) Double-flow focusing nozzle, reproduced from Oberthuer et al. (2017) under a Creative Commons Attribution 4.0 International Licence. (e) GDVN during a time-resolved experiment on PSII microcrystals, reproduced from Weierstall et al. (2012) with the permission of AIP Publishing. The white arrow shows the position of the XFEL beam and the green glow is the pump laser illumination. The outer glass tube of the GDVN was coned to allow the unrestricted passage of diffracted X-rays to large angles. All images are approximately on the same scale (scale bars are 50 µm). |