Figure 3
The two [4Fe–4S] clusters (ball-and-stick representation) in ferredoxin; the 2mFobs − DFcalc (blue, 1.0σ) and Fobs − DFcalc (green, 2.5σ) electron-density maps show indications of reproducible, localized radiation damage to heavy-atom centres in protein crystals exposed to intense XFEL radiation. The two clusters show different levels of damage, indicating that the local environment may play a role in radiation-damage dynamics at XFELs. The effects at photon energies of 7.36 and 6.86 keV (above and below the Fe K absorption edge) on the reconstructed electron-density maps of the [4Fe–4S] clusters are similar. Reproduced from Nass et al. (2015). |