Figure 3
Structure determination of the streptavidin–biotin complex by native SAD. (a) CCall versus CCweak plot, showing successful substructure determination by SHELXD. (b) Experimental phasing (2Fo − Fc) map contoured at the 1.0σ level, showing the Cα trace after autobuilding of the model with autoSHARP. (c) Cartoon representation of the secondary structure of the SavB complex in transparent grey; four S atoms (i.e. anomalous scatterers) are shown as green spheres. |