Figure 5
tNCS in the asymmetric unit found in the hRab27aMut(GppNHp) crystals. (a) Asymmetric unit composition, consisting of four tetramers (T1–T4) represented as blue and brown ribbons. (b) Superposition of the four tetramers, showing that they share the same structure. (c) Each tetramer has a noncrystallographic fourfold axis, illustrated by superimposing molecule 1 (chain A) on molecule 2 (chain B) within a tetramer. (d) Pairs of molecules between pairs of tetramers are related by twofold symmetry axes: for example chain A (from T1) and chain G (from T2). (e) The superposition of T1 and T2 tetramers related by tNCS with T3 and T4 is shown. |