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Figure 2
Structural characterization of PmScsCΔN. (a) Structure of the PmScsCΔN variant. Structural features are coloured as in Fig. 1[link] and the secondary-structure elements are labelled based on the native PmScsC structures (Furlong et al., 2017BB12). (b) Electron density of the catalytic region in PmScsCΔN. The wire mesh represents the Fo − Fc electron-density difference map (generated in phenix.refine with the region of interest omitted from the structure) contoured to 3σ and shown within a 2 Å radius of each atom. (c) Stereo diagram of the structural alignment of PmScsCΔN (green) with residues 46–221 of chain A from the compact (cyan; PDB entry 4xvw), transitional (yellow; PDB entry 5idr) and extended (magenta; PDB entry 5id4) PmScsC structures (Furlong et al., 2017BB12; r.m.s.d. of 0.51–0.68 Å, 174 residues aligned). This superimposition shows that the structures are similar whether or not the trimerization domain is present.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 75| Part 3| March 2019| Pages 296-307
https://doi.org/10.1107/S2059798319000081
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