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Figure 2
Substrate-binding site of EG27II. (a) The surface electrostatic potential of the substrate-binding cleft. Negative and positive potentials are shown in red and blue, respectively. The electron-density map (2Fo − Fc map) of the cellobiose molecules is shown at the 1.0σ level. The electrostatic potential was calculated by PyMOL. (b) The residues and waters forming hydrogen bonds to the cellobiose molecules. Hydrogen bonds are shown as dotted lines. (c) The glucose residue at subsite −1 is placed between subsites +1 and −2 by using the water positions at subsite −1. |