May 2019 issue
The policy of IUCr Journals on diffraction data is defined.
Installation of the EIGER X 1M detector onto an electron microscope and system calibration for data collection turns a transmission electron microscope into an electron diffraction instrument. Data can be collected and processed with a throughput that meets the requirements of a modern X-ray facility. The setup described here offers access to single-crystal structure determination from microcrystalline powder when large single crystals cannot be produced.
The study of coat protein assemblies on membranes by cryo-electron tomography and subtomogram averaging is discussed, outlining the methodological advancements that enabled the gain of crucial structural insight.
Phosphomannose isomerase is a zinc-dependent enzyme involved in reversible isomerization of mannose 6-phosphate and fructose 6-phosphate. Based on extensive site-directed mutagenesis, structural and biochemical studies on phosphomannose isomerase from Salmonella typhimurium, zinc ions were found to be indispensable for substrate binding and the catalytic base was identified as Lys86.
The ectodomain structure of a novel plant membrane receptor kinase with unusual capping domains is reported.
The crystal structure of the WH1 domain of the eukaryotic replication initiator protein (Rep) MSBI1.176 in the dimeric form has been determined to 1.53 Å resolution and shows a number of structural similarities to and differences from other known prokaryotic Reps.
This paper describes some statistical tools for analyses of macromolecular B values.