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![[Figure 4]](id5007fig4mag.jpg)
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Figure 4
In vitro reconstituted COPII assemblies on membranes. The addition of purified COPII components to giant unilamellar vesicles results in COPII membrane binding and the generation of a variety of morphologies, including beads-on-a-string (dashed line) and tubular (unbroken line) COPII assemblies. (a) COPII morphologies seen in a medium-magnification image of a cryo-grid. (b, c) Slices through binned 8× cryo-tomograms with 50 iterations of SIRT-like filtering applied, showing the regular lattice assembly of inner (b) and outer (c) coat components. Tomogram images are courtesy of Joshua Hutchings (unpublished work). |
![[Figure 4]](id5007fig4.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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