issue contents
May 2019 issue
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Cover illustration: The crystal structure of a transcriptional regulator's interaction with DNA displayed on an abstract depiction of the underlying data.
editorial
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The policy of IUCr Journals on diffraction data is defined.
CCP-EM
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Installation of the EIGER X 1M detector onto an electron microscope and system calibration for data collection turns a transmission electron microscope into an electron diffraction instrument. Data can be collected and processed with a throughput that meets the requirements of a modern X-ray facility. The setup described here offers access to single-crystal structure determination from microcrystalline powder when large single crystals cannot be produced.
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The study of coat protein assemblies on membranes by cryo-electron tomography and subtomogram averaging is discussed, outlining the methodological advancements that enabled the gain of crucial structural insight.
research papers
Phosphomannose isomerase is a zinc-dependent enzyme involved in reversible isomerization of mannose 6-phosphate and fructose 6-phosphate. Based on extensive site-directed mutagenesis, structural and biochemical studies on phosphomannose isomerase from Salmonella typhimurium, zinc ions were found to be indispensable for substrate binding and the catalytic base was identified as Lys86.
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The ectodomain structure of a novel plant membrane receptor kinase with unusual capping domains is reported.
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The crystal structure of the WH1 domain of the eukaryotic replication initiator protein (Rep) MSBI1.176 in the dimeric form has been determined to 1.53 Å resolution and shows a number of structural similarities to and differences from other known prokaryotic Reps.
PDB reference: MSBI1.176 WH1 domain, 6h24
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This paper describes some statistical tools for analyses of macromolecular B values.
book reviews
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obituaries
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