Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1

Hohmann, U.; Hothorn, M.

doi:10.1107/S2059798319005291

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 5
The AtSOBIR1 ectodomain is a monomer in solution. (a) Analytical size-exclusion chromatography traces of SOBIR1^1–270 (blue) and SOBIR1^1–283 (orange) with the SDS–PAGE analysis of pooled peak fractions alongside. The void volume (v₀), the total column volume (v_t) and the elution volumes for molecular-mass standards (Al, aldolase, 158 kDa; Co, conalbumin, 75 kDa; Ov, ovalbumin, 43 kDa; CA, carbonic anhydrase, 29 kDa) are indicated. (b) Analysis of the oligomeric state of AtSOBIR1. Raw right-angle light-scattering traces (blue and orange) and extrapolated molecular weights (black) of SOBIR1^1–270 and SOBIR1^1–283 are shown, with a summary table including the observed molecular weight (MW) and the dispersity (Mw/Mn) alongside. The theoretical molecular weights are 27.4 kDa for SOBIR1^1–270 and 33.3 kDa for SOBIR1^1–283.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 75| Part 5| May 2019| Pages 488-497

https://doi.org/10.1107/S2059798319005291

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