Figure 2
Sedimentation-velocity analysis of different Rubisco enzymes by analytical ultracentrifugation. Experiments were conducted using protein concentrations between 0.1 and 0.9 mg ml−1 and buffer consisting of 20 mM Tris–HCl pH 8.0, 150 mM NaCl. Where indicated, Mbu-Rubisco was dialysed against 10 mM EDTA overnight prior to loading and 10 mM EDTA was included in the subsequent buffer. Data were fitted to a continuous-size distribution model. |