Figure 5
Steady-state kinetic data for the VcCMAS enzyme. Km and Vmax determination for CTP (a), Neu5Ac (b) and Neu5Gc (c) with the VcCMAS enzyme was carried out using the EnzCheck pyrophosphatase assay (Webb, 1992). The data were fitted to the Michaelis–Menten equation using GraphPad Prism. The mean and the standard deviation were calculated from two experiments each with duplicate measurements. |