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Figure 7
Thermodynamics of the binding of CTP and CMP-sialic acid to the VcCMAS enzyme. (a) Titration calorimetry isotherms of VcCMAS with CTP and CMP-Neu5Ac, respectively. The top half of the diagram shows the heat change after each injection versus the molar protein:ligand ratio and the bottom half shows the curve fit in SEDPHAT. (b, c) Thermodynamic signatures of the binding event showing Kd, ΔH, −TΔS and ΔG in graphical and tabular form. All experiments were performed in triplicate (n = 3) and the average values are reported.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 75| Part 6| June 2019| Pages 564-577
https://doi.org/10.1107/S2059798319006831
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