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![[Figure 2]](ic5109fig2mag.jpg)
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Figure 2
Flowchart of the data-merging process. The first step for both methods is obtaining independent 3D reconstructions to calculate the scaling factor between data sets. Method 1 rescales the data at the level of micrographs and Method 2 uses extracted particles. It is essential to redo the CTF estimation (Method 1) or to apply the scaling factor to defocus values to recalculate the CTF (Method 2). Once the particles from the two data sets have been merged (`Join' job in RELION), further processing can be carried out using standard procedures. Asterisks (*) indicate where scripts are provided to perform different steps in the process: 1*, determine_relative_pixel_size.py; 2*, rescale_particles.py; 3*, scale_ctf.sh; 4*, boxscaler.py. |
![[Figure 2]](ic5109fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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