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Figure 4
Merging data sets of the polymerase module of CPF from yeast using Method 1. (a) Reconstructions of the polymerase module of CPF are shown before and after joining the data. The global and local resolution of the final 3D structure (EMDB-3908) improves after combining the data sets. Maps from data sets I and II represent the particle contribution of each data set to the final structure. Local resolution was determined using RELION for 3D reconstructions of particles from data sets I and II alone (at the final/scaled pixel size, i.e. 1.40 Å per pixel) and from combining data sets after scaling data set II using a relative pixel size of 1.28 Å per pixel. Resolutions are given according to the gold-standard criteria. (b) Fourier shell correlation plots for gold-standard refinements. (c) Example density for data sets I and II and combined data sets. The final 3D structure shows finer details in the main chain and side chains when compared with the individual data sets, as indicated by green arrows.

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ISSN: 2059-7983
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