Figure 5
Merging data sets of the yeast post-catalytic (P complex) spliceosome using Method 2. (a) The global and local resolution of the final 3D structure improves after combining the data sets. Local resolution was determined using RELION for 3D reconstructions of particles from data sets I and II alone (at the final/scaled pixel size) and from combining data sets after scaling data set II using a relative pixel size of 0.880 Å per pixel. Resolutions are given according to the gold-standard criteria. (b) Example density for data sets I and II and combined data sets. |