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Figure 6
Effect of scaling. (a) Correlation between the 3D reconstructions calculated from data set I and data set II (at 1.120 Å per pixel) using various pixel sizes for data set II. The correlation was calculated using Chimera as described in the text. (b) Gold-standard FSC for spliceosome reconstructions for data set I alone (light grey), data set II alone (black) and from combining data as described in the text (red). Additional curves come from mis-scaling data set II using the indicated pixel size to 1.120 Å per pixel, then merging with data set I, refining and post-processing. These curves are coloured the same as the corresponding points in (a). Resolutions are given according to the gold-standard criteria.

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ISSN: 2059-7983
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