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Figure 1
Example of automatic CTF selection for the 2.2 Å resolution β-galactosidase data set. Left: CTF disabled as Xmipp CTF estimates defocus U as 1.35 µm and defocus V as 1.21 µm, resulting in 0.14 µm of astigmatism in this case (less than 1% of the micrographs are disabled by this criterion). Centre: CTF disabled owing to poor visibility of the Thon rings (about 5% of the micrographs are disabled owing to this criterion). Right: CTF disabled owing to a large discrepancy between Xmipp CTF and CTFFind4 (about 5% of the micrograph are disabled owing to this criterion). After visual inspection, we realized that CTFFind4 has failed in this case.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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