Flexible workflows for on-the-fly electron-microscopy single-particle image processing using Scipion

Maluenda, D.; Majtner, T.; Horvath, P.; Vilas, J.L.; Jiménez-Moreno, A.; Mota, J.; Ramírez-Aportela, E.; Sánchez-García, R.; Conesa, P.; del Caño, L.; Rancel, Y.; Fonseca, Y.; Martínez, M.; Sharov, G.; García, C.A.; Strelak, D.; Melero, R.; Marabini, R.; Carazo, J.M.; Sorzano, C.O.S.

doi:10.1107/S2059798319011860

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
Particle-picking stage. Left: semi-automatic picking workflow, where manual picking trains the Xmipp auto-picking and fixes the particle size for EMAN2 SPARX. Right: fully automatic picking workflow using EMAN2 SPARX and Sphire-crYOLO, where the box size is estimated by Xmipp. The AND and OR consensus strategy is followed for both workflows and the resulting selections for certain micrographs from EMPIAR data set 10061, are shown.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 75| Part 10| October 2019| Pages 882-894

https://doi.org/10.1107/S2059798319011860

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