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Figure 6
Workflow example of 2D classification (purple boxes) and initial volume reconstruction (brown boxes). Since the 2D analysis is carried out on downsampled images, the resulting initial volume is resized to the original size (green box). The volume shown at the side of the workflow corresponds to the classification of 5000 particles from the 2.2 Å resolution β-galactosidase data set, which results in 17 automatically selected classes from a total of 32 (16 for CL2D and 16 for RELION 2D). In addition, Xmipp Swarm consensus has merged 21 initial volumes (ten from EMAN, ten from Xmipp Ransac and one from Xmipp Significant).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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