Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments

Andersson, R.; Safari, C.; Båth, P.; Bosman, R.; Shilova, A.; Dahl, P.; Ghosh, S.; Dunge, A.; Kjeldsen-Jensen, R.; Nan, J.; Shoeman, R.L.; Kloos, M.; Doak, R.B.; Mueller, U.; Neutze, R.; Brändén, G.

doi:10.1107/S2059798319012695

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 3
Well-based crystallization procedure. (a) A short needle is connected to the syringe to dispense the LCP suspension into the wells of a nine-well glass plate for crystallization. (b) The microcrystals are collected by pooling the LCP strings from different wells with a plunger. The LCP blob is transferred (c) from the precipitant solution to (d) a 500 µl Hamilton syringe from the back. (e) A 500 µl plunger is inserted into the syringe and slowly pushed forward to remove any residual precipitant solution. (f) A packed syringe of ba₃-type cytochrome c oxidase crystals.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 75| Part 10| October 2019| Pages 937-946

https://doi.org/10.1107/S2059798319012695

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