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![[Figure 2]](jb5013fig2mag.jpg)
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Figure 2
Biophysical characterization of the binding of the scFv to Mcl-1. Isothermal plots for the titration of the scFv (a) and Fab (b) into a solution of Mcl-1. (c) Thermal shift assay fluorescence plot versus temperature. (d) Binding sensorgrams of scFv binding to immobilized Mcl-1. Representative traces are shown (see Supplementary Figs. S2 and S3). The top concentration tested was 40 µM with a twofold dilution. (e) Steady-state fit of scFv binding to Mcl-1. |
![[Figure 2]](jb5013fig2.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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