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Figure 6
Photomicrographs showing the crystal morphologies of the samples used in structure determination and their crystallographic properties. In each case the crystallization conditions, space group, unit-cell parameters and resolution are given. (a) PDB entry 6qb3; scFv in complex with Mcl-1, apo; 10–15% PEG 200 MME, 0.1 M PCTP buffer pH 5–6; C2; a = 143.08, b = 40.38, c = 75.42 Å, α = γ = 90, β = 110.50°; resolution 1.90 Å. (b) PDB entry 6qb6; Fab in complex with Mcl-1, apo; 10–15% PEG 200 MME, 0.1 M PCTP buffer pH 5–6; C2; a = 148.05, b = 42.46, c = 106.23 Å, α = γ = 90, β = 113.19°; resolution 2.24 Å. (c) PDB entry 6qb9; scFv with bound tartrate; 1 M potassium/sodium tartrate, 0.1 M HEPES pH 7.5; C2; a = 77.88, b = 63.03, c = 101.70 Å, α = γ = 90, β = 109.96°; resolution 1.85 Å. (d) PDB entry 6qbc; Fab structure; 15%(w/v) PEG 3350, 0.1 M MgCl2, 0.1 M PCPT buffer pH 7.5; P3121; a = b = 70.54, c = 168.34 Å, α = β = 90, γ = 120°; resolution 1.56 Å. (e) PDB entry 6qf6; E. coli-scFv; 0.4 M ammonium sulfate, 25%(w/v) PEG 3350, 0.1 M bis-Tris pH 5.9; P43212; a = b = 180.18, c = 88.42 Å, α = β = γ = 90°; resolution 2.59 Å. (f) CHO-expressed scFv after treatment with carboxypeptidase Y; 0.2 M lithium chloride, 25% PEG 3350, 0.1 M bis-Tris pH 6.5. |
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