Detailed view of the hydrophobic binding cleft in the dehydrated crystal structure of afamin. A cross-section of the deep hydrophobic binding pocket (left side) is shown over transparent property-coloured residue surfaces (white, lipophilic; green, aromatic lipophilic; red, hydrogen-bonding acceptor potential; blue, hydrogen-bond donor potential). The palmitoleic acid as modelled into the cavity of PDB entry 5okl chain B (MB) is overlaid as a green ball-and-stick model. The PEG fragments modelled into the density of the dehydrated structure PDB entry 6fak (OA) are shown in a yellow ball-and-stick representation. A narrow second channel (top middle of the figure) is visible harbouring a long PEG fragment in PDB entry 6fak. Environment and electron density for the PEG fragments can be readily inspected via the PDB tools, for example https://www.ebi.ac.uk/pdbe/entry/pdb/6fak/bound/PG6. This figure was produced with ICM-Pro from Molsoft (Abagyan et al., 2006).