Figure 1
Phased anomalous difference Fourier maps and positions of the four iodine signals in L19pI-Phe crystals, displayed as purple spheres in each panel. Named and numbered residues are from Spy, whereas the partially occupied iodines are labeled I. (a) Phased anomalous difference Fourier maps from 5.2 keV data collected at two different goniometer κ angles, contoured at 5σ. (b) Phased anomalous difference Fourier map from 4.5 keV data, contoured at 4σ. (c) Phased anomalous difference Fourier maps from data collected from different crystals, contoured at 5σ (crystal 2) and 4σ (crystals 3 and 4). (d) Overlap of the L19pI-Phe iodine positions (magenta) detected here with the closest iodine signals from previous data (green; Horowitz et al., 2016). |