Comparison of the selected ensembles obtained using the current (left) and previous (right) L19pI-Phe data sets. For previously determined iodine positions, please see Supplementary Fig. S10. (a) Comparison of the READ ensembles, including the Spy (gray) and Im7 conformations (colors). (b) Contact map of the Im7–Spy interactions. The probability of contact increases from white to dark blue.