Figure 4
(a) Pro-Esp crystals. (b) Crystal structure overlay of Pro-Esp (red) and mature Esp (green). The catalytic triad and the N-terminal residues Asn73 in Pro-Esp and Val67 in mature Esp are represented as sticks in magenta. The N-terminus of Pro-Esp is repositioned, destabilizing loops 1 and 2. The oxyanion hole is in a nonfunctional conformation. (b) is enlarged to highlight the residues on the N-terminus and loops 1 and 2. |