Figure 3
GDVN used for cryo-EM grid preparation. (a) The GDVN device fitted within the current setup showing (I) the liquid inlet tubing, (II) the N2 gas inlet tubing, (III) the position of the nozzle and (IV) the grid in the target position for spraying. (b) Microscopic image of the internal GDVN geometry used in this work with the sample and gas channels labelled. The scale bar denotes 100 µm. (c) Typical grids generated with the microfluidic GDVN device under three different conditions. On all three grids, liquid was deposited approximately as a stripe across the grid. The scale bar denotes 100 µm. |