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Figure 5
The pharma Consortium on-the-fly setup consists of pushing data on the fly with Robocopy to the processing server (to an SMB-mounted folder) and simple bash scripts to extract movies (using globstar) and hard link/rename the files to .mrcs (not required for RELION-3). Scheduled RELION is then performed, usually for motion correction and CTF determination to ensure data quality, and also enables a fast start to 2D classification if desired. On-the-fly pipelines are a rapidly developing area, but in most cases some reformatting/transferring/data extraction is needed.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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