June 2020 issue
Acta Cryst. (2020). D76, 506-514
Crystal structure of an Nω-hydroxy-L-arginine hydrolase found in the D-cycloserine biosynthetic pathway
The crystal structure of DcsB, an Nω-hydroxy-L-arginine hydrolase found in the D-cycloserine biosynthetic pathway, is reported.
PDB references: DcsB at 1.5 Å, 6lug; at 1.9 Å 6luh
Acta Cryst. (2020). D76, 515-520
Based on the structure of the N-terminal region of the S97A mutant of the stomatin operon partner protein PH1510 (1510-N), it was found that the dimeric form differs greatly from those determined previously. This result indicates that 1510-N can greatly change the form of its dimer.
PDB reference: S97A mutant of 1510-N, 6m4b
Acta Cryst. (2020). D76, 521-530
Crystal structures of the glycosyltransferase PaGT3 crystallized in the presence of crown ethers provide a basis for understanding its acceptor-recognition mechanism.
PDB references: PaGT3/15-crown-5 ether, 6lzx; PaGT3/18-crown-6 ether, 6lzy
Acta Cryst. (2020). D76, 531-541
The model-building software Buccaneer has been repurposed based on existing methods for building models into cryo-EM maps. These approaches are implemented in the latest CCP-EM model-building pipeline (version 1.4.0).
Acta Cryst. (2020). D76, 542-557
Structural studies of geranylgeranylglyceryl phosphate synthase, a prenyltransferase found in thermophilic Euryarchaeota
The X-ray structure of geranylgeranylglyceryl phosphate synthase from Thermoplasma volcanium is reported, and a phylogenetic analysis and parallel ancestral state reconstruction of the evolution of the enzyme are reported for the phylum Euryarchaeota.
PDB reference: wild-type GGGPS from Thermoplasma volcanium, 6nke
Acta Cryst. (2020). D76, 558-564
High-resolution crystal structure of Trypanosoma brucei pteridine reductase 1 in complex with an innovative tricyclic-based inhibitor
Structural insights into the development of a tricyclic compound as an inhibitor of Trypanosoma brucei pteridine reductase 1 are reported.
Acta Cryst. (2020). D76, 565-580
A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis
Active human β-glucocerebrosidase was produced in baculovirus-transduced insect cells, purified and crystallized to yield a 0.98 Å resolution unliganded structure and a trapped covalent intermediate structure with 2-deoxy-2-fluoro-β-D-glucopyranoside.
Acta Cryst. (2020). D76, 581-593
The crystal structures of the PATZ1 BTB domains from mammals and fish contain homodimers. The core dimer conformation of these BTB proteins is dynamically stable, despite the presence of highly flexible regions in the dimerization interface.
PDB references: mouse PATZ1 BTB domain, 6guv; zebrafish PATZ1 BTB domain, 6guw
Acta Cryst. (2020). D76, 594-607
Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene
Dominant inheritance of hypermethioninemia is caused by an Arg264His mutation in methionine adenosyltransferase. The mutation lowers the affinity for dimer–dimer interaction and enzymatic activity, which can be restored by chemical intervention.
PDB references: human MATα1, wild type, 6sw5; R264H mutant, 6sw6
Acta Cryst. (2020). D76, 608-611
Jean-Luc Ferrer (1964–2020): structural biologist, beamline instrumentation innovator and entrepreneur
An obituary is published for Jean-Luc Ferrer, an internationally known and highly respected scientist in protein crystallography, whose work led to many innovative instrumental and methodological developments in structural biology.