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Figure 3
Purification of recombinant GBA from cell-culture medium with purification chromatograms and SDS–PAGE analyses for each purification step. GBA (∼55 kDa) was extracted from the medium by hydrophobic interaction chromatography followed by two rounds of cation-exchange chromatography with the addition of Tween 80 detergent. Purification was completed by a size-exclusion step to remove Tween 80.

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ISSN: 2059-7983
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