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Figure 7
(a) Active-site structure of the 2-deoxy-2-fluoro-β-D-glucopyranoside-GBA covalent intermediate (PDB entry 6tjq). The 2F-Glc moiety is covalently bound to the catalytic nucleophile (Glu340), which occupies two conformations. a/b, catalytic acid/base; Nuc, catalytic nucleophile; EDO, ethylene glycol. Electron density is contoured to 1.1σ (0.40 e Å−3). (c) Mechanism of the hydrolysis of 2F-DNPGlc by GBA to generate the covalent glycosyl-enzyme intermediate

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ISSN: 2059-7983
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